Påmelding til PhD Students - Atlas Antibodies AB - Internship project #1 VT25

Target group: PhD student (1 month) 

About the organization 

Atlas Antibodies is a dynamic Swedish life science company dedicated to advancing protein science and medical discovery. Founded in 2006 by researchers from the Human Protein Atlas project. The company provides over 22,000 validated primary antibodies targeting most human proteins. These high-quality reagents including PrecisA Monoclonals and Triple A Polyclonals, enable leading research in biology, pathology and medicine, contributing to improved human health globally. Atlas Antibodies is committed to innovation, sustainability, and supporting researchers worldwide in their quest for groundbreaking medical discoveries 

Project Title: NGS Single B-Cell Antibody Sequence Data Processing and Analysis 

Introduction 

Next-Generation Sequencing (NGS) of single B-cell antibody sequences provides a comprehensive understanding of the immune repertoire. This project aims to develop a robust pipeline for processing and analyzing these sequences using various bioinformatics tools. We are seeking a motivated student with relevant skills to join our team and contribute to this cutting-edge research. 

Objectives  

• Process raw NGS data: This involves assessing the quality of raw sequence data, trimming low-quality bases, and removing adapter sequences. 
• Perform quality control: Re-assessing the quality of the processed sequences to ensure data integrity. 
• Annotate and align sequences: Align sequences to reference germline V(D)J genes and annotate V(D)J gene segments, identifying CDR regions. 
• Analyze diversity and clonality: Examine clonal relationships and diversity metrics to understand the antibody repertoire. 
• Identify novel antibodies: Detect unique sequences and potential novel antibodies using analysis scripts. 

Various bioinformatics tools will be tested and finalized during the project to achieve these objectives. 

Candidate Role and Learning Opportunities 

The candidate is expected to have skills in bioinformatics, including experience with NGS data processing, and sequence alignment. Antibody sequence analysis and annotation is meritorious. The candidate will gain hands-on experience with antibody sequence data analysis and contribute to the identification of novel antibody sequences for recombinant expression. They will work closely with experienced researchers and participate in team meetings and discussions. 

Conclusion 

This project will leverage multiple bioinformatics tools to process and analyze NGS single B-cell antibody sequences, providing valuable insights into the immune repertoire and identifying novel antibodies for recombinant antibody development. The involvement of a dedicated student with relevant skills will enhance the project’s progress and provide the candidate with invaluable research experience in the industrial sector. 

Contact information 

Name: Madhuranayaki Thulasingam 

Email: madhu.thulasingam@atlasantibodies.com 

Mobile: 0703783727 
Company website: https://www.atlasantibodies.com/